Gradient Biotech

EEG connectivity

Preprocess EEG recordings, extract spectral and connectivity features, and compute graph metrics on derived connectivity matrices.

Research question

What frequency-band activity and functional connectivity patterns characterize this EEG recording, and do microstate dynamics reveal altered brain state organization?

Who this is for

  • Neurotech and digital health teams processing wearable or clinical EEG
  • Cognitive neuroscience labs studying oscillatory dynamics and functional coupling
  • Psychiatry research groups exploring EEG biomarkers for mood, attention, and sleep disorders

Data requirements

DataRequiredPurpose
EEG recordingYesPreprocessing and feature extraction
Channel montage metadataEmbedded in fileRe-referencing and bad-channel detection
Subject group labelsNo (required for cohort)Group-level feature comparison

Supported formats: EDF, BrainVision, EEGLab .set, CSV.

Workflow

Create study → Upload EEG → Preprocess → Features / microstates → Graph metrics on connectivity matrix

Step 1 — Upload EEG

Create a neurology study and upload an EEG recording from the study home page. Open the dataset workspace after upload completes.

Step 2 — Preprocess

Click Preprocess EEG. The pipeline applies:

  • Bandpass and notch filtering
  • Re-referencing (average or custom)
  • Bad-channel detection and interpolation
  • Optional ICA for artifact reduction

Review filtered recording summary, bad-channel list, and ICA component counts in the QC panel.

Step 3 — Feature extraction

Run EEG features on the completed preprocess run:

  • Band power (delta, theta, alpha, beta, gamma)
  • Spectral entropy
  • Connectivity measures: PLV, coherence, wPLI

Step 4 — Advanced EEG (optional)

Additional pipelines on preprocessed data:

PipelineOutput
MicrostatesState maps, coverage, duration, transitions, GEV
Time-frequencySpectrogram statistics, band-power dynamics
Source localizeSimplified source-space summary

Step 5 — Graph metrics on connectivity

Export or derive a connectivity matrix from PLV/coherence outputs and run Graph metrics to characterize network topology of the EEG-derived connectome.

Step 6 — Interpret (optional)

Use Interpret in microstates mode after microstate segmentation completes, or graph metrics mode after connectivity analysis.

Expected outputs

  • Preprocessed recording with QC summary
  • Band power and spectral entropy tables
  • PLV/coherence/wPLI connectivity matrices
  • Microstate maps with coverage, duration, and transition statistics
  • Graph metrics on EEG-derived connectivity
  • AI narrative citing computed spectral and connectivity values

Typical analyses

AnalysisFeatureQuestion
Alpha suppressionBand powerIs occipital alpha reduced during active attention?
Epileptiform couplingPLV connectivityAre interictal spike regions hyper-connected?
Microstate dysregulationMicrostate coverageDo patients show altered state B duration and transitions?
Wearable biomarkerTheta/beta ratioDoes the ratio differ between clinical subgroups?

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