All research areas

Computational pathology and whole-slide imaging research

Pathology

The Pathology area is a web workspace for computational pathology and whole-slide imaging research. Upload tissue slides or region images; generate tile manifests for browser viewing; run tissue detection, cell segmentation, and spatial quantification; inspect overlays in the slide viewer; and track reproducible pipeline runs.

Use cases

Start from a research scenario

Guided workflows map common questions to data requirements, analysis steps, and documentation — pick the scenario closest to your study.

All Pathology use cases

Slide quantification

Research question: What is the tissue composition of this slide, how many cells are detected, and what spatial metrics characterize the tissue architecture?

Run the full computational pathology pipeline on a tissue slide — tile generation, tissue detection, cell segmentation, and spatial quantification with interactive viewer review.

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TME infiltration

Research question: How infiltrated is the tissue, what is the spatial organization of detected cells, and does the infiltration pattern suggest immune-desert, excluded, or inflamed phenotypes at the tissue level?

Quantify tumor microenvironment architecture — cell density patterns, immune infiltration phenotype, and spatial proximity metrics from segmented tissue slides.

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Annotated compartments

Research question: What are the cell counts, densities, and spatial statistics within each labeled tissue compartment rather than across the whole slide?

Quantify cell density and spatial metrics within pathologist-defined regions — tumor, stroma, necrosis, or lymphoid compartments imported from GeoJSON or QuPath exports.

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Spatial omics context

Research question: Where do spatial gene expression signals originate relative to tissue structure, and how can morphology-guided regions connect molecular data to what pathologists see on the slide?

Ground spatial transcriptomics in tissue morphology — pathology provides the WSI tissue context layer that makes Visium, Xenium, and MERFISH results interpretable in histological space.

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Capabilities

Platform features

Everything available in the Pathology workspace today — pipelines, explorers, exports, and provenance.

  • Study containers for whole-slide imaging and region-image projects
  • Slide upload for TIFF, OME-TIFF, PNG, JPEG, and scanner-format files
  • Multi-level tile generation with thumbnail and pyramid manifests for web viewing
  • Tissue detection producing masks, region bounding boxes, and focus QC summaries
  • Cell and nucleus segmentation with overlay PNG and structured cell tables
  • Spatial quantification: cell density heatmaps across tissue regions
  • Nearest-neighbor distance statistics between detected cells
  • Infiltration phenotype scoring and region-level metric summaries
  • CSV and JSON exports for downstream statistical analysis
  • Interactive slide viewer with pan/zoom tile rendering
  • Viewer layers for tissue mask, cell overlay, and density heatmaps
  • GeoJSON and QuPath-compatible annotation import
  • Study-level pipeline run history with linked artifacts
  • Cohort comparison of pathology biomarkers across sample groups
  • Reproducible pipeline versioning recorded on every analysis run
  • Async pathology job dispatch with queued, running, and complete states
  • Research-oriented outputs with explicit non-diagnostic disclaimers