Gradient Biotech

Glossary

TermDefinition
AI interpretationGrounded narrative summary (cluster annotation, DE, enrichment, or study/methods text) generated from structured run results; cannot cite values absent from the run payload
AnnDataAnnotated data matrix format (.h5ad) used internally for all modalities
CheckpointIntermediate processed .h5ad written between pipeline steps
ContrastA saved comparison between two groups (for example treatment vs control)
Coverage DEGMulti-class biomarker step ranking genes by recurrence across all pairwise class comparisons
DEDifferential expression — genes that change between groups or clusters
DeconvolutionEstimating cell-type composition fractions from sample- or spot-level expression profiles
Disease evidenceCurated gene-disease association lookup applied to run-derived gene sets; research context, not diagnostic evidence
DomainSpatial cluster of spots representing a tissue region
EnrichmentStatistical over-representation or ranking of genes against curated pathways (GO, KEGG)
ExperimentDatabase/API name for a study
GSEAGene set enrichment analysis — ranked whole-DE-table enrichment method, reported alongside ORA
HVGHighly variable gene — selected before dimensionality reduction
IntegrationBatch-correction step that removes sample/batch-driven structure from a joint PCA/clustering
LeidenGraph-based clustering algorithm used after neighbor graph construction
MetadataSample or cell annotations merged into AnnData obs
ModalityAssay type: single-cell, bulk, spatial, or biomarker
NicheLightweight spatial candidate region nominated from co-localizing, enriched tissue domains
ORAOver-representation analysis — enrichment method for thresholded gene lists
Pipeline runOne execution of an analysis step with saved parameters and results
ProvenanceRecord of how a result was produced (parameters, versions, inputs)
PseudobulkAggregating single-cell counts by sample and cell type/cluster, then running replicate-aware DE
PseudotimeGraph-based ordering of cells along an inferred trajectory
QCQuality control — filtering low-quality cells or samples
RunSee pipeline run
Single-cell CNVChromosome-scale copy-number inference from single-cell expression, used for malignant-cell and subclone calls
SketchRepresentative-cell downsampling and projection strategy for working with large single-cell datasets
SnapshotFrozen study state referencing specific runs and parameters
ssGSEASingle-sample gene set enrichment — per-sample or per-cell gene-set scores
StaleResult outdated because upstream data or parameters changed
StudyTop-level project container in the UI
SVGSpatially variable gene — expression varies across tissue coordinates
TrajectoryOrdering of cell states (pseudotime) plus cell-cycle scoring for differentiation/activation analysis
UMAP2D embedding for visualizing cell similarity
WGCNAWeighted gene co-expression network analysis — modules, hub genes, and eigengene-trait correlations
WilcoxonNon-parametric test used for rank-based DE in the current pipeline